diff --git a/docs/api/user-event.md b/docs/api/user-event.md
index 04e4375c7..7991ec3f3 100644
--- a/docs/api/user-event.md
+++ b/docs/api/user-event.md
@@ -293,3 +293,32 @@ The sequence of events depends on whether the scroll includes an optional moment
 - `momentumScrollBegin`
 - `scroll` (multiple events)
 - `momentumScrollEnd`
+
+## `accessibilityAction()`
+
+```ts
+accessibilityAction(
+  instance: TestInstance,
+  actionName: string,
+): Promise<void>
+```
+
+Example
+
+```ts
+const user = userEvent.setup();
+await user.accessibilityAction(slider, 'increment');
+```
+
+Simulates an assistive technology (e.g. a screen reader) triggering the named accessibility action on a given element, invoking its `onAccessibilityAction` handler.
+
+The `actionName` autocompletes the [standard React Native actions](https://reactnative.dev/docs/accessibility#accessibility-actions) (`activate`, `increment`, `decrement`, `longpress`, `magicTap`, `escape`, `expand`, `collapse`), but any custom action name is accepted.
+
+Just like a real assistive technology, the action must be reachable by the user, otherwise an error is thrown:
+
+- the action must be declared in the element's `accessibilityActions` prop,
+- the element must not be disabled (via `aria-disabled` or `accessibilityState={{ disabled: true }}`).
+
+### Sequence of events
+
+- `accessibilityAction`
diff --git a/src/event-builder/common.ts b/src/event-builder/common.ts
index 43b6606ae..9224faa0c 100644
--- a/src/event-builder/common.ts
+++ b/src/event-builder/common.ts
@@ -66,3 +66,19 @@ export function buildBlurEvent() {
     },
   };
 }
+
+/**
+ * Builds an accessibility action event, as delivered to the `onAccessibilityAction`
+ * handler when an assistive technology triggers an action.
+ *
+ * Experimental values:
+ * - `{"actionName": "increment"}`
+ */
+export function buildAccessibilityActionEvent(actionName: string) {
+  return {
+    ...baseSyntheticEvent(),
+    nativeEvent: {
+      actionName,
+    },
+  };
+}
diff --git a/src/user-event/accessibility-action/__tests__/accessibility-action.test.tsx b/src/user-event/accessibility-action/__tests__/accessibility-action.test.tsx
new file mode 100644
index 000000000..8421504d6
--- /dev/null
+++ b/src/user-event/accessibility-action/__tests__/accessibility-action.test.tsx
@@ -0,0 +1,94 @@
+import * as React from 'react';
+import type { AccessibilityActionEvent } from 'react-native';
+import { View } from 'react-native';
+
+import { render, screen, userEvent } from '../../..';
+import { createEventLogger, lastEventPayload } from '../../../test-utils/events';
+
+async function renderViewWithActions(props: React.ComponentProps<typeof View> = {}) {
+  const { events, logEvent } = createEventLogger();
+
+  await render(
+    <View
+      testID="view"
+      accessible
+      accessibilityActions={[{ name: 'increment' }, { name: 'activate', label: 'Activate' }]}
+      onAccessibilityAction={(event: AccessibilityActionEvent) =>
+        logEvent('accessibilityAction')(event)
+      }
+      {...props}
+    />,
+  );
+
+  return { events };
+}
+
+describe('userEvent.accessibilityAction', () => {
+  test('triggers the onAccessibilityAction handler with the given action name', async () => {
+    const user = userEvent.setup();
+    const { events } = await renderViewWithActions();
+
+    await user.accessibilityAction(screen.getByTestId('view'), 'increment');
+
+    expect(events).toHaveLength(1);
+    expect(events[0].name).toBe('accessibilityAction');
+    expect(lastEventPayload(events, 'accessibilityAction').nativeEvent).toEqual({
+      actionName: 'increment',
+    });
+  });
+
+  test('supports the direct (setup-less) call form', async () => {
+    const { events } = await renderViewWithActions();
+
+    await userEvent.accessibilityAction(screen.getByTestId('view'), 'activate');
+
+    expect(events).toHaveLength(1);
+    expect(lastEventPayload(events, 'accessibilityAction').nativeEvent.actionName).toBe('activate');
+  });
+
+  test('throws when passed a non-host instance', async () => {
+    const user = userEvent.setup();
+    await renderViewWithActions();
+
+    // @ts-expect-error intentionally passing a non-host instance
+    await expect(user.accessibilityAction('not a host instance', 'increment')).rejects.toThrow(
+      /works only with host instances/,
+    );
+  });
+
+  test('throws when the action is not declared in accessibilityActions', async () => {
+    const user = userEvent.setup();
+    await renderViewWithActions();
+
+    await expect(user.accessibilityAction(screen.getByTestId('view'), 'decrement')).rejects.toThrow(
+      /has no "decrement" accessibility action.*"increment", "activate"/s,
+    );
+  });
+
+  test('throws when the element declares no accessibility actions', async () => {
+    const user = userEvent.setup();
+    await renderViewWithActions({ accessibilityActions: undefined });
+
+    await expect(user.accessibilityAction(screen.getByTestId('view'), 'increment')).rejects.toThrow(
+      /has no accessibility actions/,
+    );
+  });
+
+  test('throws when the element is disabled', async () => {
+    const user = userEvent.setup();
+    await renderViewWithActions({ 'aria-disabled': true });
+
+    await expect(user.accessibilityAction(screen.getByTestId('view'), 'increment')).rejects.toThrow(
+      /on a disabled element/,
+    );
+  });
+
+  test('throws when the element is disabled via accessibilityState', async () => {
+    const user = userEvent.setup();
+    await renderViewWithActions({ accessibilityState: { disabled: true } });
+
+    await expect(user.accessibilityAction(screen.getByTestId('view'), 'increment')).rejects.toThrow(
+      /on a disabled element/,
+    );
+  });
+});
diff --git a/src/user-event/accessibility-action/accessibility-action.ts b/src/user-event/accessibility-action/accessibility-action.ts
new file mode 100644
index 000000000..bfa40c6fd
--- /dev/null
+++ b/src/user-event/accessibility-action/accessibility-action.ts
@@ -0,0 +1,84 @@
+import type { AccessibilityActionInfo } from 'react-native';
+import type { TestInstance } from 'test-renderer';
+
+import { buildAccessibilityActionEvent } from '../../event-builder';
+import { computeAriaDisabled } from '../../helpers/accessibility';
+import { isTestInstance } from '../../helpers/component-tree';
+import { ErrorWithStack } from '../../helpers/errors';
+import type { StringWithAutocomplete } from '../../types';
+import type { UserEventInstance } from '../setup';
+import { dispatchEvent } from '../utils';
+
+/**
+ * Standard accessibility action names recognized by React Native (`activate`,
+ * `increment`, `decrement`, `longpress`, `magicTap`, `escape`, `expand`,
+ * `collapse`). Custom action names are supported as well, hence the `string`
+ * fallback.
+ *
+ * @see https://reactnative.dev/docs/accessibility#accessibility-actions
+ */
+export type AccessibilityActionName = StringWithAutocomplete<
+  | 'activate'
+  | 'increment'
+  | 'decrement'
+  | 'longpress'
+  | 'magicTap'
+  | 'escape'
+  | 'expand'
+  | 'collapse'
+>;
+
+/**
+ * Simulate an assistive technology (e.g. screen reader) triggering an
+ * accessibility action on a given element.
+ *
+ * This will call the `onAccessibilityAction` handler with an event carrying the
+ * given `actionName`.
+ *
+ * Like a real assistive technology, the action must be declared in the element's
+ * `accessibilityActions` prop, and the element must not be disabled. Otherwise an
+ * error is thrown.
+ *
+ * @param instance element to trigger the action on
+ * @param actionName name of the accessibility action to trigger
+ */
+export async function accessibilityAction(
+  this: UserEventInstance,
+  instance: TestInstance,
+  actionName: AccessibilityActionName,
+): Promise<void> {
+  if (!isTestInstance(instance)) {
+    throw new ErrorWithStack(
+      `accessibilityAction() works only with host instances.`,
+      accessibilityAction,
+    );
+  }
+
+  const actions = instance.props.accessibilityActions as
+    | ReadonlyArray<AccessibilityActionInfo>
+    | undefined;
+
+  if (!actions?.length) {
+    throw new ErrorWithStack(
+      `The element has no accessibility actions. Add them using the "accessibilityActions" prop.`,
+      accessibilityAction,
+    );
+  }
+
+  if (!actions.some((action) => action.name === actionName)) {
+    const available = actions.map((action) => `"${action.name}"`).join(', ');
+    throw new ErrorWithStack(
+      `The element has no "${actionName}" accessibility action. Available actions: ${available}.`,
+      accessibilityAction,
+    );
+  }
+
+  if (computeAriaDisabled(instance)) {
+    throw new ErrorWithStack(
+      `Cannot trigger the "${actionName}" accessibility action on a disabled element.`,
+      accessibilityAction,
+    );
+  }
+
+  await dispatchEvent(instance, 'accessibilityAction', buildAccessibilityActionEvent(actionName));
+}
diff --git a/src/user-event/accessibility-action/index.ts b/src/user-event/accessibility-action/index.ts
new file mode 100644
index 000000000..c84e7b3d9
--- /dev/null
+++ b/src/user-event/accessibility-action/index.ts
@@ -0,0 +1 @@
+export { accessibilityAction, AccessibilityActionName } from './accessibility-action';
diff --git a/src/user-event/index.ts b/src/user-event/index.ts
index d2d0a9622..b19451cdf 100644
--- a/src/user-event/index.ts
+++ b/src/user-event/index.ts
@@ -1,5 +1,6 @@
 import type { TestInstance } from 'test-renderer';
 
+import type { AccessibilityActionName } from './accessibility-action';
 import type { PressOptions } from './press';
 import type { ScrollToOptions } from './scroll';
 import { setup } from './setup';
@@ -20,4 +21,6 @@ export const userEvent = {
   paste: (instance: TestInstance, text: string) => setup().paste(instance, text),
   scrollTo: (instance: TestInstance, options: ScrollToOptions) =>
     setup().scrollTo(instance, options),
+  accessibilityAction: (instance: TestInstance, actionName: AccessibilityActionName) =>
+    setup().accessibilityAction(instance, actionName),
 };
diff --git a/src/user-event/setup/setup.ts b/src/user-event/setup/setup.ts
index 336d19f2a..feb75d32d 100644
--- a/src/user-event/setup/setup.ts
+++ b/src/user-event/setup/setup.ts
@@ -3,6 +3,8 @@ import type { TestInstance } from 'test-renderer';
 import { jestFakeTimersAreEnabled } from '../../helpers/timers';
 import { validateOptions } from '../../helpers/validate-options';
 import { wrapAsync } from '../../helpers/wrap-async';
+import type { AccessibilityActionName } from '../accessibility-action';
+import { accessibilityAction } from '../accessibility-action';
 import { clear } from '../clear';
 import { paste } from '../paste';
 import type { PressOptions } from '../press';
@@ -153,6 +155,21 @@ export interface UserEventInstance {
    * @returns
    */
   scrollTo: (instance: TestInstance, options: ScrollToOptions) => Promise<void>;
+
+  /**
+   * Simulate an assistive technology (e.g. screen reader) triggering an
+   * accessibility action on a given element.
+   *
+   * The action must be declared in the element's `accessibilityActions` prop and
+   * the element must not be disabled, otherwise an error is thrown.
+   *
+   * @param instance element to trigger the action on
+   * @param actionName name of the accessibility action to trigger
+   */
+  accessibilityAction: (
+    instance: TestInstance,
+    actionName: AccessibilityActionName,
+  ) => Promise<void>;
 }
 
 function createInstance(config: UserEventConfig): UserEventInstance {
@@ -168,6 +185,7 @@ function createInstance(config: UserEventConfig): UserEventInstance {
     clear: wrapAndBindImpl(instance, clear),
     paste: wrapAndBindImpl(instance, paste),
     scrollTo: wrapAndBindImpl(instance, scrollTo),
+    accessibilityAction: wrapAndBindImpl(instance, accessibilityAction),
   };
 
   Object.assign(instance, api);
diff --git a/website/docs/14.x/docs/api/events/user-event.mdx b/website/docs/14.x/docs/api/events/user-event.mdx
index 198dc0c13..ce9b153b7 100644
--- a/website/docs/14.x/docs/api/events/user-event.mdx
+++ b/website/docs/14.x/docs/api/events/user-event.mdx
@@ -294,3 +294,32 @@ The sequence of events depends on whether the scroll includes an optional moment
 - `momentumScrollBegin`
 - `scroll` (multiple events)
 - `momentumScrollEnd`
+
+## `accessibilityAction()`
+
+```ts
+accessibilityAction(
+  instance: TestInstance,
+  actionName: string,
+): Promise<void>
+```
+
+Example
+
+```ts
+const user = userEvent.setup();
+await user.accessibilityAction(slider, 'increment');
+```
+
+Simulates an assistive technology (e.g. a screen reader) triggering the named accessibility action on a given element, invoking its `onAccessibilityAction` handler.
+
+The `actionName` autocompletes the [standard React Native actions](https://reactnative.dev/docs/accessibility#accessibility-actions) (`activate`, `increment`, `decrement`, `longpress`, `magicTap`, `escape`, `expand`, `collapse`), but any custom action name is accepted.
+
+Just like a real assistive technology, the action must be reachable by the user, otherwise an error is thrown:
+
+- the action must be declared in the element's `accessibilityActions` prop,
+- the element must not be disabled (via `aria-disabled` or `accessibilityState={{ disabled: true }}`).
+
+### Sequence of events
+
+- `accessibilityAction`